Membrane Requirements For Uridylylation Of The Poliovirus VPg Protein And Viral RNA Synthesis In Vitro

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Requirements for Assembly of Poliovirus Replication Complexes

(36, 52). It also catalyzes the uridylylation of VPg (viral protein 3B) in vitro, in the presence of poly(A) (42). A short unpaired nucleotide sequence in a highly conserved stem-loop formed by

Membrane Requirements for Uridylylation of the Poliovirus VPg

Membrane Requirements for Uridylylation of the Poliovirus VPg Protein and Viral RNA Synthesis In Vitro Mark H. Fogg,† Natalya L. Teterina, and Ellie Ehrenfeld*

Dissertation

positive-strand RNA synthesis 4.141 The cloverleaf structure is required in the positive- strand for positive-strand RNA synthesis. 4.142 Binding-sites for PCBP2 and 3CDpro in the cloverleaf are required for positive-strand RNA synthesis. 4.143 Constructs with two cloverleaf structures support cre(2C) mediated VPg-uridylylation.