Determination Of Vascular Permeability

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Venous occlusion pressure and vascular permeability in the

an increase in upstream resistance and vascular permeability, venous occlusion pressure did not increase, and marked edema did not occur. pulmonary vascular resistance; pulmonary edema; isolated lung lobe AIR EMBOLIZATION is a potentially lethal consequence of a number of clinical situations (8, 9, 25, 35, 38).

Pulmonary Vascular Permeability and Ischemic Injury in

Address correspondence to: Patrice M. Becker, Division of Pulmonary For determination of baseline pulmonary vascular permeability, and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, 5- to 6-wk-old male C57BL/6 (wild-type) or gelsolin / mice (weight 5501 Hopkins Bayview Circle, Baltimore, MD 21224. E-mail: [email protected]


Feb 18, 2013 vascular endothelial growth factor to inhibit angiogenesis and vascular permeability. 1. Ranibizumab may be indicated when ALL of the following are present: 1.1 Age 18 years or older 1.2 No concurrent ocular or periocular infection 1.3 Eye condition appropriate for ranibizumab treatment, as indicated by 1 or more of the following: a.

CHAPTER 3 Treatment - WHO

increase in vascular permeability leading to loss of plasma from the vascular compartment. Studies reveal a reduction in plasma volume of more than 20% in severe cases. The evidence that supports the existence of plasma leakage includes findings of pleural effusion and ascites by examination or radiography,

PEDF Improves Cardiac Function in Rats with Acute Myocardial

Mar 11, 2015 cardiomyocytes, enhancement of vascular permeability, myocardial fibrosis and left ventricular remodeling. Indeed, cardiomyocyte apoptosis and vascular permeability are involved in myocardial fibrosis and LV remodeling, thus promoting contractile dysfunction [2]. After these effects, irreversible damage occurs in the ischemic heart.

Quantitative Fluorescence Microscopy Measures Vascular Pore

3. The permeability surface area product (PS) can be substituted for Kin from eq. E, as the measured Kin for all tracers are permeability limited with values that are significantly lower than 20% of cerebral vascular blood flow. 4. That a (solute radius) is at least 6-fold smaller than p (vascular pore radius). Cancer Research

Fraxin Alleviates LPS-Induced ARDS by Downregulating

Vascular Permeability Xiaohong Ma,1,2 Xiangyong Liu,1,4 Jiali Feng,1,2 Dong Zhang,1,2 Lina Huang,1 Dongxiao Li,1,2 Liang Yin,3 Lan Li,1 and Xiao-Zhi Wang2 Abstract Acute respiratory distress syndrome (ARDS) is a severe acute disease that threatens human health, and few drugs that can effectively treat this disease are available.

Determination of vascular permeability coefficients under

Determination of vascular permeability coefficients under slow luminal filling James G. Truslow, Joe Tien⁎ Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA article info abstract Article history: Accepted 12 July 2013 Available online 23 July 2013

Effect of Lung Irradiation on the Incidence of Pulmonary

The procedure for determination of vascular permeability (the extravasation rate of plasma protein) was almost the same as that described by SONG & LEVITT (1971). Whole blood (0.5 ml) taken from a normal mouse, was incubated with 30 pCi of sodium 51Cr-chromate for 60 min in a water bath of 37°C and unbound 51Cr was

RESEARCH ARTICLE Open Access Presence of intratumoral

performed by Westernblotting and immunohistochemical staining. Vascular permeability was evaluated by determination of intratumoral Evans blue and Miles vascular permeability assay. Furthermore, microdialysis was used to examining the intratumoral extracellular angiogenic growth factors (VEGF, TGF-β) by ELISA.


vascular permeability [12, 13]. It is reported that tryptase may contribute to vascular permeability by the direct or indirect generation of bradykinin from kininogens [14]. Mast cell tryptase increases intracellular Ca2+, leading to elevation of paracellular permeability of colonocytes [15]. Intradermal injection of tryptase or mast cell

Enhancement of vascular permeability by specific activation

Enhancement of vascular permeability by specific activation of protease-activated receptor-1 in rat hindpaw: a protective role of endogenous and exogenous nitric oxide *,1Atsufumi Kawabata, 1Ryotaro Kuroda, 2Hiroyuki Nishikawa, 3Toshiharu Asai, 3Kazuo Kataoka & 3Mamoru Taneda

REVIEW Open Access Pathophysiology and biomarkers of acute

mandated determination of the PaO 2/FIO 2 ratio under positive airway pressure. However, no new parameters or biomarkers were adopted. In this review, the current understanding of the basic ARDS pathophysiology and associated candidate biomarkers will be discussed. Review Regulation of vascular permeability

Simultaneous perfusion and permeability measurements using

corrected vascular permeability parameters Ktrans and v e, based on a reference-tissue model18 without determination of an arterial input function. However, they did not determine leakage-corrected, quantitative DSC PWI maps. The present study is based on a modified version of the approach used by Bjørnerud et al.14 Instead of relying on single-

Simultaneous determination of blood flow, microvascular

Simultaneous determination of blood flow, microvascular permeability and blood volume in lung cancer overexpressing different VEGF isoforms in a murine xenograft model by dynamic contrast enhancement MR imaging (DCE-MRI) C-M. Shih1,2, A. Yuan 3, C-Y. Chen2, H-W. Cheng , T-W. Chou 2, P-C. Yang3, J-H. Chen1, and C. Chang 2

In vivo studies on the actions of platelet-activating factor

2.5 In vivo determination of nasal vascular permeability inthe guinea-pig 2.5.1 Animal preparations 63 2.5.2 Measurement of vascular permeability 65 Chapter 3- A comparison between acoustic rhinometry and posterior rhinomanometry in the measurement of nasal airway patency. 3.1 Introduction 6 6 3.2 Experimental protocol 69

Effect of Sialoadenectomy on Stomach Lesions Induced by

For the determination of gastric vascular permeability we used Evans blue (Fluka Chemia AG) and the method described by Udaka et al. (1970) and Szabo et al. (1985). The Evans blue was injected into the tail vein in a dose of 20 1 as a 0.5 % solution and 50 min later, after extraction (Lacy et al. 1990), its

CURRICULUM VITAE: Joe Y. Tien Boston University Boston, MA

39. Truslow, J.G. & Tien, J., Perfusion systems that minimize vascular volume fraction in engineered tissues. Biomicrofluidics 5, 022201 (2011). 38. Price, G.M. & Tien, J. Methods for forming human microvascular tubes in vitro and measuring their macromolecular permeability. In Biological Microarrays (Methods in Molecular Biology,

Recombinant Tissue Factor Pathway Inhibitor Reduces

: TFPI and Pulmonary Vascular Injury 1753 weight ratio, and coagulopathy was estimated by measuring serum con-centrations of fibrin and fibrinogen degradation products E (FDP[E]). Control animals received saline instead of the study drugs. Determination of pulmonary vascular permeability index. 125 I-la-beled bovine serum albumin (125


cervical vascular changes. Some of the proposed regulators of cervical microvasculature include sensory neuronal-derived factors, such as Substance P (SP). Collins et al., (2000) observed that SP in rats induced microvascular changes, such as increased vascular permeability, increased white blood cells (WBC) infiltration and vasodilatation. We have

Rat VEGF quantikine

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and in adults (1-3). It is a member of the PDGF family that is characterized by the presence of

MRI Estimation of the Arterial Input Function in Mice1

and/or vascular permeability within various tissues. The pharmacokinetic models most commonly employed for analysis of DCE-MRI data require knowledge of the time-dependent tracer concentration in the vessels feeding the tumor, known as the arterial input function (AIF) (1 3). The AIF has previously been determined in humans

Ozurdex (dexamethasone intravitreal implant)

capillary leakage and phagocytic migration. Corticosteroids inhibit the expression of vascular endothelial growth factor (VEGF), a cytokine that is a potent promoter of vascular permeability and expressed at increased concentrations in macular edema. Dexamethasone is a corticosteroid which is five times more potent than triamcinolone acetonide

PAF increases vascular permeability without increasing

Determination of Pulmonary Microvascular Permeability EBD technique. EBD, 6,69-[(3,39-dimethyl[1,19-biphenyl]-4, 49-diyl)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid], a nonradioactive diazo dye that binds tightly to albumin, is a sensitive marker for early pulmonary edema (30, 36). Pro-longed perfusion with EBD does not alter

Synergistic effects of EMPs and PMPs on pulmonary vascular

vascular permeability was increased (Fig. 1e, f). In addi-tion, large amount of inammatory cells leaked into the interstitial space of lung (Fig. 1g, h) and the protein con-centration in bronchoalveolar lavage uid (BALF) was signicantly increased in I/R rats (Additional le 2: Fig. S1B). Furthermore, H/R-treated pulmonary VECs were

The role of oxygen-derived free radicals in ischemia-induced

increases skeletal muscle vascular permeability (McNamee and Grodins, 1975; Korthuis et al., 1984a). Thus, the purpose of this study was to evaluate the role of oxygen-derived free radicals and histamine as putative mediators of ischemia-induced increases in skeletal muscle vascular permeability. The osmotic reflection coefficient and

Local Coverage Determination for Vascular Endothelial Growth

Vascular endothelial growth factor (VEGF) plays an important role in both physiologic and pathologic angiogenesis and contributes to increased permeability across both the blood-retinal and blood-brain barriers. VEGF is a protein that stimulates the growth, proliferation, and survival of vascular endothelial cells. After two decades of

Lack of IL-6 augments inflammatory response but decreases

BBB permeability index was calculated by dividing the value for each sample by the mean value of the control animals. Mouse intracerebral injection and determination of BBB disruption Determination of the effect of IL-6 on cerebral vascular permeability was performed as previously described (Paul et al., 2001). Recombinant human IL-6 (Biosource,

Effect of Allopurinol and Superoxide Dismutase on

For the determination of gastric vascular permeability we used Evans blue (Fluka Chemia AG) according to the method described by Szabo et al. (1985). The Evans blue was injected into the tail vein (30 min after indomethacin) in a dose of 20'1 as a 0.5 % aqueous solution. Thirty minutes later, under

p38 MAP Kinase a molecular switch between VEGF‐induced

Determination of vascular permeability in vitro Hydraulic conductivity was determined as described previously (28). VEGF (1 ng/ml) and/or 10 µM SB203580, respectively, or solvent were applied to the upper chamber, and hydraulic conductivity was assessed continuously in the presence of 10 cm hydrostatic pressure. Because

SUMMARY JN121 - Food and Drug Administration

assessment of the extent and type of perfusion, blood volume and vascular permeability changes-Dynamic Diffusion Analysis -Generates apparent diffusion coefficient maps used for the visualization of apparent water movement in soft tissue throughout the body on both voxel-by-voxel and sub-voxel bases.

Comparison of Cerebral Blood Volume and Vascular Permeability

Ktrans, a parameter related to vascular permeability, was calculated from a two-compartment pharmacoki-netic model (24). Both of these parameters were measured from the same dynamic, susceptibility-weighted, contrast-enhanced (DSC) MR imaging data. Although rCBV and vascular permeability have already been correlated with glioma grade, determin-

Covalent dimerization of vascular permeability factor

Vascular permeability factor, or vascular endothelial growth factor (VPFNEGF) is an important factor in the regulation of vascular growth and vascular permeabil- ity. VPF is a secreted, dimeric protein and has 8 cysteine residues conserved with platelet-derived growth factor (PDGF).

Activation of Sphingosine Kinase-1 Reverses the Increase in

endothelial permeability and does so by the activation of endothelial cell S1P1 signaling pathway. Materials and Methods Animals, cell culture and transfection, RT-PCR, SPHK activity assay, S1P measurement, confocal imaging, endothelial permeability determination, and lung vascular permeability determination are

Tiam1andRac1AreRequiredforPlatelet-activating Factor

vascular permeability could be obtained without a direct involvement of any contractile mechanism (11 16). The main component of the vascular barrier, the ECs, has more than 10% of their total protein represented by actin (17), which under physiological salt concentrations subsists as monomers(G-actin)andassembledintofilaments(F-actin).A

Quantitative Evaluation of Vascular Permeability in the

tive and morphological evaluation of vascular permeability in animal tissues. J Neurosci Methods 8:41-49 Sztriha L, J60 F, SZerdahelyi P, Eck E, Koltai M (1986) Effect of dexamethasone on brain edema induced by kainic acid sei­ zures. Neuroscience 17:107-114

Local Coverage Determination (LCD): Vascular Endothelial

Vascular endothelial growth factor (VEGF) plays an important role in both physiologic and pathologic angiogenesis and contributes to increased permeability across both the blood-retinal and blood-brain barriers.1 VEGF is a protein that stimulates the growth, proliferation, and survival of vascular endothelial cells. After two decades of extensive

RESEARCH Open Access Vascular endothelial growth factor

vascular permeability. Thus, VEGF is a potential candidate for genetically influencing AMD susceptibility, based on its functional relevance to AMD pathophysiology. VEGF gene exhibits many single nucleotide polymor-phisms (SNPs) that might influence qualitatively and/or quantitatively its expression. Previous studies have shown

Human VEGF Immunoassay

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight

Determination of the Bone Lacunar Canalic ular Permeability

permeability of bone as a function of loading frequency. An accurate determination of the PLC permeability is critical to better understand the mechanotransduction mechanism in bone. ACKNOWLEDGEMENTS: This work was financially supported by grants from the NIH/NIA (AG34198), NSF (PHY-0848491) and NSF/MRI (CBET 0723027).