Log Molecular Weight Calculator Protein

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BCHM 461 Exam #3 Problem 1. (27 points total)

BCHM 461 Exam #3 p.2 Problem 1. (27 points total) a. (5 points) A protein has binding affinity for its ligand (a peptide) of Ka = 2 10 5 M-1 at pH 5.0 and 25oC.At what concentration of the ligand is half of the protein bound?

Extinction Coefficients - ResearchGate

A = -log T = log (1/T) In a spectrophotometer, monochromatic plane-parallel light enters a sample at right angles to the plane-surface of the sample. 10 = (εpercent) × (molecular weight of

REFERENCE GUIDE FOR PHARMACEUTICAL CALCULATIONS

* Each gm of protein/aminoacid provides = 4 calories / kcal * Each gm of medium chain triglyceride (mct) = 8.3 calories / kcal * Each gm of glycerol provides = 4.3 calories / kcal * Each cc of alcohol provides = 5.6 calories / kcal * 1 cc of 10% fat emulsion provides = 1.1 calories / kcal * 1 cc of 20% fat emulsion provides = 2.0 calories / kcal

Gel Filtration - Harvard University

according to differences in their molecular size. High resolution fractionation can be used to isolate one or more components, to separate monomers from aggregates, to determine molecular weight or to perform a molecular weight distribution analysis. Gel filtration can also be used to facilitate the refolding of denatured proteins by careful

Molecular Weight Estimation - Bio-Rad

Fig. 2. Determining the MW of an unknown protein by SDS-PAGE. A standard curve of the log (MW) versus R f was generated using the Precision Plus Protein standards from Figure 1. The strong linear relationship (r2 > 0.99) between the proteins MW and migration distances demonstrates exceptional reliability in predicting MW. Molecular Weight

Tech Tip 0015 - A Guide to Understanding Extinction Coefficients

The molar absorption coefficient of a peptide or protein is related to its tryptophan (W), tyrosine (Y) and cysteine (C) amino acid composition. At 280 nm, this value is approximated by the weighted sum of the 280 nm molar absorption coefficients of these three constituent amino acids, as described in the following equation:3,4

Protein Marker, Broad Range (2-212 kDa)

Protein Marker, Broad Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 13 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250 (1).

Assay Validation Methods - Definitions and Terms

Repeatability expresses the precision under the same operating conditions over a short interval of time. Repeatability is also termed intra-assay precision. Intermediate Precision expresses within-laboratories variations: different days, different analysts,

blotting - Bio-Rad

Plus Protein WesternC standards provide a robust and direct method for protein MW estimation from western blots. sample buffer (Bio-Rad catalog #161-0737) from the 1 mg/ml stock solutions to final protein amounts of 200, 150, 100, 75, 50, 25, 12.5, 6.2, 3.1, and 1.5 ng per 5 µl gel sample load.

International System of Units (SI Units)

Growth Hormone Binding Protein pmol/L 1.0 pmol/L hCG, Beta Subunit mIU/mL 1.0 IU/L hCG, Total mIU/mL 1.0 IU/L 5-HIAA mg/24 h 5.23 μmol/d mg/g creat 0.592 μmol/mmol Histamine Plasma or whole blood ng/mL 8.997 nmol/L Urine μg/g creat 1.018 nmol/mmol creat mg/24 h 8.997 μmol/d Homovanillic Acid mg/24 h 5.49 μmol/d

Appendix C: Water Activity and Isotherm Equations

s is the weight ration of each solute to the total of solids in the mixture, and M s is the molecular weight of each component (Fontan et al. 1981). ISOTHERM EQUATIONS 1. BET isotherm: where m is the moisture in g/100 solids or g/g solids at water activity a w and m o is the monolayer value in same units. The constant c is calculated by:

Quest Diagnostics Units of Measure (UOM)

log 10 : log to the base 10 Log copies/mL : logarithmic copies per milliliter Log IU/mL : logarithmic international units per milliliter Log titer : logarithmic titer mcg : microgram mcg E1/g stool : microgram of estrone per gram of stool mcg/100 g : microgram per 100 grams mcg/12 h : microgram per 12 hours mcg/2 h : microgram

Calculations involving concentrations , stoichiometry

relative atomic (molecular) weight Molar Volume one mole of any gaseous substance occupies the same volume at the same temperature and pressure 22.414 litres at 101.325 kPa, 0 °C (273.15 K) (Avogadro s Law)

Technical Fact Sheet N-Nitroso-dimethylamine (NDMA)

Yellow liquid with no distinct odor (ATSDR 1989; Cal/EPA 2006) Yellow liquid with faint, characteristic odor (NIOSH 2010; HSDB 2012). Molecular weight (g/mol) 74.08

TR0006.4 Introduction

) × (molecular weight of protein) Still other sources provide protein absorbance values for 0.1% (= mg/mL) solutions, as this unit of measure is more convenient and common for protein work than percent solution. This variation in reporting style underscores the importance

Lecture 13 Electrophoresis (Part-I)

molecular weight (due to SDS they have equal negative charge). After tracking dye reaches to the bottom of the gel, gel is taken out from the glass plate with the help of a spatula. Gel is stained with coomassie brilliant blue R250 dye. The dye stains protein present on the gel. A typical SDS-PAGE pettern is given in the Figure 13.6.

POLYMER END-GROUP ANALYSIS: THE DETERMINATION OF AVERAGE

average molecular weight of a polymer. The weight average molecular weight, Mw, is given by where W i is the weight of polymer with molecular weight M i. Here each M is multiplied by the weight present in the sample, and the sum of all these contributions is divided by the total weight of the sample.

Basic Concepts in Pharmacokinetics - Warwick

Relationship of PK parameters ( ) CL ln 2 V t 2 1 ⋅ = The elimination half-life is defined as the time for the drug concentration to reach half of its value.

CARB COUNTER - Atkins

Kay s Naturals Protein Apple Cinnamon, dry 1.2 oz 12.0 LC Foods Cereal, Raspberry, dry C 1.5 Nutlettes, dry C 4.0 Nutritious Living Hi-Lo, Original,dry 1/2 C 7.0 Sensato High Fiber Butter Pecan, prepared 1/2 C 1.0 Cinnamon, dry 1/2 C 2.0 Dixie Diners Carb Counters Meal in a Cup 1 C 5.0 Mac & Cheese 1 C 3.0 Spaghetti 1 C 5.0 Teriyaki

Physicochemical properties of drugs in solution

gram equivalent weight, is analogous to the mole or gram molecular weight. When monovalent ions are considered, these two terms are identical. A 1 molar solution of sodium bicarbonate, NaHCO3, contains 1 mol or 1 Eq of Na! and 1 mol or 1 Eq of HCO per litre (dm03) of solution. With multivalent ions, attention must be paid to the valency of

Lesson 3: Calculations used when compounding medications

concentration and a desired final drug amount (in either weight or volume) to determine the amount of each individual ingredient to add. Just like that. If you cook, you ve probably done proportional calculations, even if you cook using only a can-opener and a microwave. Red Alert!! An important message regarding proportional calculations.

Ligand efficiency indices for effective drug discovery

a key property of a compound is its molecular weight (MW), a natural extension of the concept of ligand efficiency has been introduced recently that is based on expressing the binding affinity as pK i = -log K i and using MW as reference expressed in kDa (Box 1 of [12]). Three related efficiency indices: per cent efficiency index (PEI); binding

Humate-P Dosage Calculator - CSL Behring

VWF:RCo activity was 2.4 IU/dL per IU/kg (range: 1.1 to 4.2). High molecular weight multimers were measured in 13 subjects with type 3 VWD; 11 had absent or barely detectable multimers at baseline. Of those 11 subjects, all had some high molecular weight multimers present 24 hours after infusion of Humate-P

Materials Characterization by Thermal Analysis (DSC & TGA

Molecular weight distribution and branching Crystallinity Crystallite morphology as determined by thermal history Differences affect end-use performance-20-15-10-5 0 Heat Flow (W/g) 40 60 80 100 120 140 160 Exo Up Temperature (°C)

Diafiltration: A Fast, Efficient Method for Desalting, or

As a general rule, the molecular weight cut-off (MWCO) of the membrane should be 1/3rd to 1/6th the molecular weight of the molecule to be retained (3-6X Rule). This is to assure complete retention. The closer the MWCO is to that of the sample, the greater the risk for some small product loss during concentration. The risk increases if

Ultraviolet Light Disinfection Data Sheet

Jun 17, 2013 The reduction of micro-organisms is classified using a logarithmic scale. A single log reduction is a 90% reduction of organisms. A two log reduction is a 99% reduction of organisms, followed by a three log reduction (99.9%), etc. The UV-C exposure dosage needed for each level of reduction is shown in the

Guidelines for Intravenous Albumin Administration at Stanford

Mar 15, 2017 May be used in demonstrated nephrotic syndrome (>3 g/day of urinary protein excretion [or spot protein equivalent] + hypercholesterolemia + hypoalbuminemia) and loop diuretic resistance (defined as an insufficient response to an intravenous bolus dose of ≥160 mg furosemide or 4 mg bumetanide

Diffusion measurements by Nuclear Magnetic Resonance (NMR)

molecular displacement. Apart form the pioneering works mentioned above, a number of reviews articles and books on the basic methodology have appeared [5,6,7,8]. 3

REVISED METHOD FOR DETERMINATION OF ACID VALUE IN OILS AND

g) The weight of the oil/fat taken for the estimation and the strength of the alkali used for titration shall be such that the volume of alkali required for the titration does not exceed 10mL. 11.7 Calculation: Acid value = 56.1 V N W Where, V = Volume in mL of standard potassium hydroxide or sodium hydroxide used

How to use a protein assay standard curve

A protein assay, such the BCA Protein Assay, is an excellent tool for estimating the protein concentration of a sample. The intensity of the colored reaction product is a direct function of protein amount that can be determined by comparing its absorbance value to a standard curve.

Drug Design: Functional groups / Pharmacological Activity

molecular weight less than or equal to 500 calculated logP less than or equal to 5 *Compound classes that are substrates for biological transporters are exceptions to the rule. Not too polar Not too big Not too hydrophobic

Dynamic Light Scattering: An Introduction in 30 Minutes

the log of the relative scattering intensity versus particle size at angles of 173o (the detection angle of the Zetasizer Nano S and Nano ZS in aqueous media) and 90o (the detection angle of the Nano S90 and Nano ZS90) assuming a laser wavelength of 633nm, real refractive index of 1.59 and an imaginary refractive index of 0.001.Mie theory is

Modeling Drug Passage into Breastmilk

Ion Trapping Rasmussen F. In, Brodie BB, Gillette JR, eds. Concepts in biochemical pharmacology. New York: Springer -Verlag 1971:390-402.

3.4 TEST FOR BACTERIAL ENDOTOXINS Final text for revision of

∑ e = the sum of the log endpoint concentrations of the dilution series used f = the number of replicate test tubes The geometric mean endpoint concentration is the measured sensitivity of the lysate (IU/ml). If this is not less than 0.5λ and not more than 2λ, the labeled sensitivity is confirmed and is used

Foundation GPC Polymers and Molecular Weight

Molecular Weight 9 The molecular weight of a polymer is a way of describing how long the polymer chains are Each monomer has a molecular weight (often called the formula weight) Adding the monomers together to make polymers increases the molecular weight The longer the chains, the higher the molecular weight

Choosing Right Column for Reverse Phase HPLC Separations

Size-exclusion columns separate proteins based on molecular weight, or more accurately, hydrodynamic volume. Ideally, the surface of the chromatography media should not interact with the protein, a requirement difficult to meet for a wide variety of proteins. ZORBAX GF-250 and ZORBAX GF-450 columns are manufactured to

Viscosity - Moodle USP: e-Disciplinas

Plotting log[η] vs. logM will easily give K and a for that polymer-solvent pair. Many K s and a s for common polymer-solvent pairs can be found in handbooks of polymer science. Once K and a are known, a measurement of [η] on a polymer of unknown molecular weight can be used to measure the molecular weight.

[ Care and Use ManUal ] - Waters Corporation

characterize proteins ranging in molecular weight range from 10,000 450,000 daltons including monoclonal IgG mono-mers and their aggregates. The BEH125 SEC, 1.7 µm offering is best suited for the analysis of peptides and proteins in the molecular weight range from 1,000 - 80,000 daltons (Figure 1).

Lecture 1 Fundamentals of Protein Chemistry and Mass Spectrometry

If you measure the weight of a peptide with 100 carbon atoms, 98.89% of the carbons will have an atomic weight of 12, and 1.11% will have an atomic weight of 13.003354 The average atomic weight of carbon is 12.0111 The monoisotopic molecular weight is 12.0000