Neuronal Growth And Differentiation On Biodegradable Membranes

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Biomaterials for Enhancing Neuronal Repair

cues, and stiffness in biomaterials to enhance neuronal dynamics (e.g., neuritogenesis), improve growth, and allow monitoring of neural systems. Cues or properties are compared for relative impact on neuronal behavior and development (Table1). While the integration of chemical cues into materials has been widely

Proliferating Cells versus Differentiated Cells in Tissue

within a Petri dish or on a scaffold. Growth factors and fetal bovine serum are employed to drive cultured cells from one mitosis to the next as quickly as possible. The tissue specific interphase is extremely short under these conditions, so that the degree of differentiation desired in tissue engineering cannot be achieved.

Biomedical and Therapeutic Applications of Biosurfactants

differentiation.59 MEL has been implicated in the induction of neuronal differentiation in PC12 cells and therefore provides the basis for the use of glycolipids as therapeutical agents for cancer treatment. Nevertheless, further studies of the molecular basis of the signalling cascade that follows


the endogenous expression of multiple growth factors and simultaneously modulate stem cell differentiation. Our previous study showed that knocking down miR-31 promotes the osteogenesis of bone marrow stromal stem cells (BMSCs). To investigate the therapeutic potential of cells engineered to express anti-miR-31 for CSD repair,

OPEN Electrospun SF/PLCL nanofibrous membrane: a potential

inal neuronal cells. In general, the direct injection of a cell suspension using a needle leads to poor cell survival and migration due to the shearing forces induced during cell injection and reflux17. By contrast, biodegradable polymer scaffolds can deliver these cells to the subretinal space in a more organised man-

High-Throughput Screening of a Small Molecule Library for

differentiation paths of delivered or interacting cells. The assessment of the proper chemical cues has also been extensively investigated, and can be divided between the use of either growth factors or the use of small molecules. Growth factors can naturally drive cell differentiation, such as during development and wound repair, through interac-

Biomedical Applications of Hydrogels Handbook

The applications of electroconductive hydrogels as biorecognition membranes for implantable biosensors, as electro-stimulated drug-eluting devices and as a low interfacial impedance layer on neuronal prostheses present new horizons for biodetection devices.

Potent tumor tropism of induced pluripotent stem cells and

no neuronal or glial differentiation. iPSCs or iPS-NSCs labeled with 5-bromo-2-deoxyuridine were intracranially implanted in the contralateral hemisphere to the GL261 glioma cell implantation in the allogeneic C57BL/6 mouse. Active migration of both stem cells was observed 7 days after implantation. Again, the iPSCs located in the tumor area

Substrates, Matrices & Scaffolds - AMSBIO

endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound repair. Natural Extracellular Matrices ECM proteins have revolutionized in vitro and in vivo cell models by providing optimal environmental conditions to

Stem Cell Therapies in Obstetrics and Gynaecology: The Female

growth factor secreting feeder function to stimulate regeneration. Nevertheless, the concept of stem cell injection for sphincteric muscle regeneration is the subject of research in a number of centres and the development of improved forms of treatment for SUI might yet prove to be one of the major clinical benefits of regenerative medicine.


mary neuronal cell growth is under intense investigation, including evaluating polyelectrolyte (PE) membranes as sub-strates for neuronal cell growth.3 5 One type of membrane is made of graphene and its derivatives; its effect on the behav-ior and differentiation of neuronal and stem cells was

RESEARCH Open Access Effects of collagen membranes enriched

cells undergo neuronal differentiation in response to dimethylsulfoxide (DMSO), adenosine 3 ,5 -cyclic mono-phosphate (cAMP), or serum withdrawal [38-43,36,37,12-14]. Upon induction of differentiation, proliferation of N1E-115 cellsceases, extensive neurite outgrowth is observed and the membranes become highly excitable [38-43,36,37,12-14].

Enhancement of neural stem cell survival, proliferation and

repair bone defects.25 Therefore, GO can not only deliver growth factors but also protect their biological activity. The electrical properties of GO are also bene cial to NSC neuronal differen-tiation and axonal regeneration. Thus, in this study, we reported graphene oxide-incorporated PLGA nano bre membranes with immobilised

Nanomembranes and Nanofibers from Biodegradable Conducting

proliferation or differentiation of various cell types. The first sections of the review are focused on a general overview of conducting and biodegradable polymers most usually employed and the explanation of the most suitable techniques for preparing nanofibers and nanomembranes (i.e., electrospinning and spin coating). Following sections are

Microtube array membrane bioreactor promotes neuronal

stones have been reached in 3D neuronal constructs, which are able to mitigate neuronal injuries, many hurdles need to be faced and solved. Most of the dynamic devices are aimed to promote neuronal expansion and differentiation but are lacking of the ability to drive cell elongation according to a precise pattern[13 16].

General Information - FenixEdu


Biological Performance of Electrospun Polymer Fibres

induce neuronal growth and guide brain regeneration [13]) and the treatment of pulmonary diseases (e.g., porous scaffolds that mimic alveolar units to allow for greater cell adhesion for lung tissue regeneration [14]). Studies involving the testing of cellular response against electrospun scaffolds have been have

Biomedical Research 2017; 28 (6): 2411-2426 -DTPA-bis (N

Apoptosis plays an important role in cellular growth, differentiation, homeostasis, and the regulation of immune function and removal of damaged cells. So, dysfunction and dysregulation of apoptosis results in pathological damages. Defects in apoptosis can give rise to cancer, autoimmune diseases and spread of viral infections, whereas increased

Electrophoresis 1 Research Article 2 3 Photo-crosslinkable

The differentiation of stem cells is significantly affected by various extracellular microenvironments [17]. To manip-ulate stem cell fate, various multifunctional microfluidic devices have recently been developed [18 22]. For instance, compartmental microfluidic culture device has been de-veloped to induce neuronal differentiation from murine

Biosurfactants: potential applications in medicine

* induction of cell differentiation in the human promyelocytic leukemia cell line HL60 * induction of neuronal differentiation in PC12 cells Rhodococcus erythropolis treahalose lipid * antiviral activity against HSV and influenza virus 89, 90 Streptococcus thermophilus glycolipid * anti-adhesive activity against several bacterial and yeast strains


perivascular areas, sub-amniotic and amniotic membranes. The efficiency of hMSC derivation from UCB by various workers has been very low compared to hWJSCs and UCB-MSC colonies have slow growth reaching confluence only after 20 days in culture compared to the much shorter time of 5 to 6 days taken for hWJSCs.

Advanced Drug Delivery Reviews - Nanomedicinelab

CNTs and their ability to cross biological membranes. Next, biodegrada-tion of CNT is considered, a critical issue for any delivery system. The eventual fate of nanoparticles following the transport of therapeu-tics or diagnostics to target sites will also determine their clinical adoption. Recent studies have shown that CNTs can be biodegradable

Neuronal growth and differentiation on biodegradable membranes

Neuronal growth and differentiation on biodegradable membranes Sabrina Morelli1, Antonella Piscioneri1, Antonietta Messina1,3, Simona Salerno1, Mohamed B. Al-Fageeh2, Enrico Drioli1,3,4 and

Surface Modification for Improve Biocompatibility of

herein was permissive for neuronal growth and experimental differentiation in vitro. Devices constructed from the bulk material had acceptable handling properties and performed comparatively with an autograft control in a thy-1-YFP-H mouse 3 mm gap injury model after 21 days, with the number of unique axons at the distal end in each repair

Theanine-Modified Graphene Oxide Composite Films for Neural

growth medium for two days, and then, the growth medium was removed and replaced to differentiation medium. Cell differentiation assay included immunofluorescence staining and real-time PCR, which was performed after cell cultured for7d.Forimmunofluorescencestaining,thecellswerefixed with 4% paraformaldehyde for 30min, extracted with 0.1%

New frontiers in biomaterials research for tissue repair and

neuronal growth using rat hippocampal neurons. Growth of these cells in culture, accompanied by functional synapse formation, was observed. In order to comprehensively and extensively understand this field, Aggarwal et al.[10] reviewed the structure, synthesis, and assembly of central nervous system (CNS) myelin. In the CNS, oligodendrocytes

Cellular Systems and Biomaterials for Nerve Regeneration in

Cellular Systems and Biomaterials for Nerve Regeneration in Neurotmesis Injuries 417 cell adherence was more difficult to obtain and imply the previous covering with poli-l-


various scaffold and growth factors on MSC differentiation and proliferation pathways will need to be evaluated. P-86: Biodegradable Polymer Modified Magnesium-Based Scaffold for Bone Tissue Engineering K. Wong 1, P. Chu2, K. Luk , K. Cheung1, K. Yeung1; 1Orthopaedics and Traumatology, The University of Hong Kong,

Development of Self-Assembled Nanoribbon Bound Peptide

to promote axonal growth and to bridge neural ending defects [18]. For instance, it was reported that composites of poly -caprolactone electrospun membranes and gelatin improved cell adhesion, proliferation and differentiation of PC-12 nerve cells and supported neurite outgrowth [19]. In a recent

Periodontal Tissue Engineering Strategies Based on Nonoral

growth/differentiation factors or filler materials and guided tissue regener-ation. However, these treatments have come short on achieving regenera-tion ad integrum of the periodontium, mainly due to the presence of tissues from different embryonic origins and their complex interactions along the regenerative process.

LEI CAI - Stanford University

Functionalized biomaterials with bioactive peptides to enhance neuronal cell growth. Developed injectable, biodegradable polymeric scaffolds for bone and nerve tissue engineering. 2006 - 2008 Undergraduate Thesis, Polymer Science & Engineering, Zhejiang University. Advisor: Qiaoling Hu, Ph.D.

Available online at www.sciencedirect

S. Morelli et al. / Procedia Engineering 44 ( 2012 ) 363 366 365)LJ 6(0¶V LPDJHV RI 6+6< < FHOOV DIWHU GD V RI FXOWXUH RQ 3&/ 38 PHPEUDQHV

Evaluation of biocompatibility of random or aligned

engineering showed that PHB membranes induced neural progenitor cells and embryonal cell lines to neuronal differentiation (Xu et al., 2010; Khorasania et al., 2011). PHB scaffolds have been used as a guidance channel in in vivo studies. PHB scaffolds supported nerve survival and axonal regeneration in both spinal cord and peripheral

Electrospun PU scaffolds for proliferation and neuronal

Apr 29, 2020 a neuronal differentiation medium consisting of Neurobasal A basal medium supplemented with 1% B27 and 1% N2 (all media components from GibcoBRL/Invitrogen), omitting the growth factor bFGF. For long-term differentiation culture (18 47 days) 20 ng ml−1 of epidermal growth factor (EGF) and bFGF was added to the differentiation medium. The


34 Nanofiber membranes with controllable microwells and structural cues and their use in forming cell microarrays and neuronal networks Xie J, Liu W, MacEwan MR, Yeh YC, Thomopoulos S and Xia Y, Small, 2011, 7, 293-297. (This article was featured on the cover.) (citation: 9 times)

Kristan S. Worthington

Kristan S. Worthington 1402 Seamans Center Iowa City, IA 52242 (319)-335-5632 [email protected] CURRENT POSITION Assistant Professor Biomedical Engineering, University of Iowa Aug 2017 Present


supported the differentiation and extension of neurites in a direc-tion parallel to the long axis of the fiber. However, the fibrous scaffold was confined in the agarose gel, restricting the flexibility of further engineering parameters as compared to self-standing fibers. Here, we present a facile approach to exploit genetically

Nano Plasma equine - Nu-Vet

PMVs may also promote neuronal cell proliferation, survival, and differentiation, suggesting a role in treating brain injuries. PMVs can be internalized by brain endothelial cells in vitro, and can modify their phenotype and functions. PMVs, which promote all stages of angiogenesis in vivo, in addition to

ISSN 2284-6808 Letters in Applied NanoBioScience

biodegradable material with varied tissue engineering applications. PCL along with Hydroxyapatite (HA) [7], PCL-gelatin hybrid nanofibrous membranes [8], PCL-(Poly-1,4- butylene adipate-co-polycaprolactam HA scaffold [9], are used in bone tissue engineering. However, for tissue engineering application hydrophobic

Cellular Systems and Biomaterials for Nerve Regeneration in

Upon induction of differentiation, proliferation of N1E-115 cells ceases, extensive neurite outgrowth is observed and the membranes become highly excitable (Koka & Hadlock, 2001; Luis et al., 2007b; Luis et al., 2008). The ideal interval period of 48 hours of differentiation