Dual SgRNA‐directed Gene Deletion In Basidiomycete Ganoderma Lucidum Using The CRISPR/Cas9 System

Below is result for Dual SgRNA‐directed Gene Deletion In Basidiomycete Ganoderma Lucidum Using The CRISPR/Cas9 System in PDF format. You can download or read online all document for free, but please respect copyrighted ebooks. This site does not host PDF files, all document are the property of their respective owners.

Applications and Challenges of the Most Popular Gene

by C Tastan 2020 Deleting the ORAI1 gene in immune T cells in the laboratory with CRISPR gene targeted using the CRISPR-Cas9 system containing modified sgRNA to reduce 

2015 APS Annual Meeting Abstracts of - CGSpace

by JF Hernández Nopsa 2015 genes were quantified 0, 2, and 4 days after inoculation (DAI) using qPCR. Infection of the root system occurs prior to the onset of.

Mind the mushroom: natural product biosynthetic genes and

6 Jan 2021 lism, Liu et al. succeeded in deleting a gene in Ganoderma lucidum, using a dual sgRNA-directed CRISPR-Cas9 system.23 Pre-.

Efficient genome editing with CRISPR/Cas9 in - AMB Express

by T Boontawon 2021 Cited by 2 in fcy1 via homology-directed repair using this CRISPR/Cas9 system was also efficiently introduced (sgRNA), which results in the cleavage of genomic DNA.11 pages

27th Fungal Genetics Conference - Genetics Society of

achieved via the dual functions of these molecules as adhesins as well as signaling A CRISPR/Cas9 system for genetic engineering of filamentous fungi.264 pages

Біотехнологія ХХІ століття - Кафедра біотехніки та

21 Apr 2017 D. TARGETED GENE INTEGRATION IN PLANTS USING THE López-Arredondo D.L. A dual fertilization and weed control system based on genetically.

directed gene deletion in basidiomycete Ganoderma

by K Liu 2020 Cited by 12 Dual sgRNA-directed gene deletion in basidiomycete. Ganoderma lucidum using the CRISPR/Cas9 system. Ke Liu. †. Bin Sun. †. Hao You, Jun-Liang Tu, Xuya Yu,.

Poster Abstracts - Sociedad Mexicana de Bioquímica

Transcriptional regulatory networks integrate gene regulation as directed links Kinetic characterization of the radial growth of Ganoderma lucidum on.

PDF Plus - APS Journals - American Phytopathological Society

genes were quantified 0, 2, and 4 days after inoculation (DAI) using qPCR. primers directed the amplification of the expected size ~1.1 kb fragment and.

Title Efficient genome editing with CRISPR/Cas9 in Pleurotus

by T Boontawon 2021 Cited by 2 An NHEJ-mediated gene mutation using the. CRISPR/Cas9 system typically produces small inser- tions or deletions (indels), such as the insertion  12 pages

IMC'18 Program 070418 1129AM - British Mycological Society

16 Jul 2018 expression of a number of protease genes were obtained using a biological in vivo in the CRISPR/Cas9 system in the filamentous fungus 

CRISPR-Cas系统编辑丝状真菌的进展与挑战 - 合成生物学

by X Han Dual sgRNA-directed gene deletion in basidiomycete Ganoderma lucidum using the. CRISPR/Cas9 system[J]. Microbial Biotechnology, 2020,. 13(2): 386-396.

БИОТЕХНОЛОГИЯ: СОСТОЯНИЕ И ПЕРСПЕКТИВЫ

SALT STRESS ON GANODERMA LUCIDUM: MORPHOLOGICAL, PHYSIOLOGICAL AND BIOCHEMISTRY DEVELOPMENT OF CRISPR/CAS9 SYSTEM FOR GENOME EDITING IN DEBARYOMYCES.

2018 International Symposium and Annual Meeting of

14 Jun 2018 Systemic dual gene expression in pepper using a broad bean wilt virus 2-based vector system. Boram Choi1, Soo-Jung Han2, Jang-Kyun Seo1,2*.

1-s2.0-S0734975019300369-main - University of Helsinki

by RS Kun 2019 Cited by 25 In order to perform genetic manipulations with the CRISPR/Cas9 system, both the Cas9 nuclease and the sgRNA need to be present in the host  42 pages

High-throughput targeted gene deletion in the model - Nature

by PJ Vonk 2019 Cited by 18 Various efficient approaches of genome editing using CRISPR/Cas9 have been reported in the model fungus. Saccharomyces cerevisiae. For example, cas9 and sgRNAs 

Abstract download (PDF) - Asian Mycological Congress 2019

In addition, Ganoderma lucidum, Phellinus spp.,. Agaricus brazei and Cordyceps spp. have been cultivated to medicinal mushrooms. Recently, the funtional.

БІОЛОГІЇ - Faculty of Biology - Львівський національний

by МІ ПОСТУП The CRISPR-Cas9 system uses small RNA molecules (crRNA and tracrRNA) DysDf//TM6,Tb containing deletion of the dystrophin gene was used.

Donnloaded from: www.agrobiosonline.com

along with CRISPR Cas 9 gives opportunity to alter the gene expression by fusing transcriptional deletion by using TALEN (Transcription activator-.

Identifizierung, Expression und Charakterisierung

by CJ Behrens 2018 In contrast to other known laccases a deletion of up to 13 amino acids in this region Establishing the CRISPR-Cas9 system for the use of Basidiomycota.

www.bio-protocol.org/e3796 Dual sgRNA-based Targeted

by Y Jin 2020 [Abstract] CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in  17 pages

Filamentous fungi for protein production and - DTU Orbit

by JKH Rendsvig 2020 Using our gene expression platform, we investigate the Efficient CRISPR-Cas9 systems have been developed for filamentous fungi,  250 pages

CRISPR system in filamentous fungi - ScienceDirect.com

by H Deng 2017 Cited by 53 Keywords: CRISPR/Cas9 system, filamentous fungi, drug discovery, sgRNA nucleotides target genomic DNA via Watson-Crick base pairing, and Cas9 can.

Transcriptional Dynamics of Genes Purportedly - MDPI

by M Cai 2021 Abstract: Ganoderma lucidum spores (GLS), the mature germ cells gene silencing systems [8] and dual sgRNA-directed gene deletion.

Recent developments of tools for genome and metabolome

by F Alberti 2020 Cited by 3 the scaffold upon which natural product gene clusters can be predicted. CRISPR/Cas9-based engineering of basidiomycete fungi has been.12 pages